MDMAPR - Molecular Detection Mapping and Analysis Platform
Runs a Shiny web application that merges raw 'qPCR'
fluorescence data with related metadata to visualize species
presence/absence detection patterns and assess data quality.
The application calculates threshold values from raw
fluorescence data using a method based on the second derivative
method, Luu-The et al (2005) <doi:10.2144/05382RR05>, and
utilizes the ‘chipPCR’ package by Rödiger, Burdukiewicz, &
Schierack (2015) <doi:10.1093/bioinformatics/btv205> to
calculate Cq values. The application has the ability to connect
to a custom developed MySQL database to populate the
applications interface. The application allows users to
interact with visualizations such as a dynamic map,
amplification curves and standard curves, that allow for
zooming and/or filtering. It also enables the generation of
customized exportable reports based on filtered mapping data.